Journal: Scientific reports
Article Title: A functional IL1RL1 variant regulates corticosteroid-induced sST2 expression in ulcerative colitis.
doi: 10.1038/s41598-017-10465-0
Figure Lengend Snippet: Figure 1. Corticosteroid treatment increases serum levels of ST2 in UC. Serum ST2 levels in UC patients grouped according to therapies (A) or according to disease score activity and corticosteroid (CORT) treatment (B) were determined by ELISA. 5-ASA (5-aminosalicylic acid), AZA (azathioprine), corticosteroids (hydrocortisone, prednisone and prednisolone), IFX (infliximab). Differences were assessed using the Kruskal- Wallis test and Dunn’s multiple comparison post-test. *p < 0.05; **p < 0.01; ***p < 0.001. sST2 (C), IL6 (D) in conditional media of biopsies from healthy controls (HC) or UC patients, stimulated or not with 100 nM Dexametasone (Dex) for 24 hours. Differences between medians were assessed using the Mann-Whitney U-test. *p < 0.05; **p < 0.01.
Article Snippet: A mouse monoclonal antibody against human ST2 (MAB523, R&D Systems) and an Alexa 546-tagged secondary goat antibody against mouse IgG (Invitrogen/Life Technologies, Carlsbad, CA, USA) were used.
Techniques: Activity Assay, Enzyme-linked Immunosorbent Assay, Comparison, MANN-WHITNEY